Introduction: At the international level, Directly Observed Treatment Short-Course (DOTS) is a public health strategy to effectively fight Tuberculosis (TB) epidemic. However, because of the irrational use of anti-tuberculosis drugs, Mycobacterium tuberculosis is developing more and more resistance to antibiotics and the number of cases of multidrug-resistant Tuberculosis (MR-TB and XDR-TB) is increasing. Thus, the therapeutic arsenal used in this context becomes limited and the care also becomes difficult.
Objective: The study aims to demonstrate in vitro efficacy of MATHESIA on Mycobacterium tuberculosis.
Methods: Thirty eight (38) positive Ziehl or Auramine sputum from failures and relapses cases were collected. After treatment with 4% sodium hydroxide, the direct method of proportion technique was used. It consisted of inoculating the pellets on Löwenstein-Jensen's medium (LJ), on incorporated LJ of MATHESIA at different concentrations and on LJ incorporated anti-tuberculosis drugs with well-known critical concentrations. The tubes were incubated at 37°C and read at 28 and 42 days for susceptibility testing.
Results: Six strains (15.8%) were sensitive to all the anti- TB drugs tested and also to MATHESIA. In contrast, 32 strains (84.2%) were resistant to at least one antituberculosis drug. In contrast to MATHESIA, all the 32 strains resistant to at least one antituberculous drug showed growth only on a single concentration (0.01 mg/ml) whereas in the other 4 concentrations, growths were not observed, even for 21 MDR-TB souches (55.3%).
Conclusion: The anti-mycobacterial activity is certain in the MATHESIA and more in-depth research must be done to determine its behavior in the treatment.
Kabedi Bajani Marie Jose, Kayembe Ntumba Jean Marie, Kashongwe Munogolo Zacharie, Bisuta Fueza Serge, Mampasi Kusangabo Philippe, Mbaya Kalumba Paulin, Taba Kalulu M, Mifundu MN, Mulenga Mbombo C, Nkasa HL, Tshitadi Makangu Augustin, Nganga Nkanga Mireille and Muyembe Tamfum Jean Jacques